This document serves as the user manual for the Izon Automatic Fraction Collector V2 (AFC). It provides comprehensive instructions for setting up, operating, and maintaining the device. The AFC automates the collection of fractions from qEV size-exclusion columns, increasing uniformity of collected volumes and decreasing experimental run-time.
The manual covers essential aspects such as safety precautions, instrument specifications, assembly instructions, and detailed operating procedures. It also includes guidance on choosing appropriate run parameters, troubleshooting common issues, and updating the AFC firmware. The AFC measures the weight of each fraction to determine its volume and ensures uniform volumes across all fractions. Calibration of the scale is performed using the supplied 10 g weight.
The AFC is designed for indoor use only and should be operated within the specified environmental conditions. Users are advised to follow all safety guidelines and precautions to prevent potential hazards. The manual also provides information on disposal of biohazardous materials and decontamination procedures.